It is a high-throughput-friendly, cost-effective alternative to electroporation.Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific) was used for comparison. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use. Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. TransIT-X2® Transfection Reagent Enables Superior Transfection in a Variety of Cell Types.TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios.Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Gene editing detection assay. Cell line-specific transfection conditions using Lipofectamine CRISPRMAX Reagent. Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of … The following cell line-specific conditions are provided as a starting point for transfecting wild-type cells with TrueGuide Synthetic gRNA and TrueCut Cas9 Protein v2 using the Lipofectamine CRISPRMAX Transfection Reagent. Transfection of RNP complexes was performed using the Lipofectamine CRISPRMAX Reagent (Invitrogen). Further optimization of the transfection conditions may be necessary for best results. siRNA was … ... in experiments using the doxycycline-inducible system. Gene editing efficiency was measured using a Guide-it Mutation Detection Kit (Takara Bio). Cells were harvested, washed with 1× PBS, and lysed after 48 hours of treatment unless otherwise specified.

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